Biochar-immobilized Sphingomonas sp. and Acinetobacter sp. isolates to enhance nutrient removal: potential application in crab aquaculture
Biochar-immobilized Sphingomonas sp. and Acinetobacter sp. isolates to enhance nutrient removal: potential application in crab aquaculture
Blog Article
The frequency of water exchange and reducing ribavirin coupon the risk of eutrophication to surrounding water bodies have always been water-quality control issues in recirculating aquaculture systems.In this study, maize straw biochar prepared through pyrolysis showed great potential for both bacterial immobilization and pollutant adsorption.Heterotrophic bacterial strains of Sphingomonas sp.PDD-57b-25 and Acinetobacter towneri were isolated in situ from wastewater for pollutant remediation through a 16S rDNA-based method, which has been rarely reported to date.The selected strains had higher ammonia nitrogen (NH4+-N, 63%), nitrite nitrogen (NO2--N, 38%), nitrate nitrogen (NO3--N, 25%) and total phosphorus (TP, 35%) assimilation capacities than those of other widely applied bacteria under similar medium conditions.
In addition, more NH4+-N (+16%), NO2--N (+14%), NO3--N (+17%) and TP (+19%) was removed by biochar-immobilized isolated strains than dissociated strains, suggesting their use may provide a means of improving water-quality control in recirculating aquaculture.With specific additions (4 g l-1) of biochar-immobilized Sphingomonas sp.PDD-57b-25 and A.towneri, the dissolved inorganic nitrogen (approximately 0.45 mg l-1) and TP (approximately 0.
09 mg l-1) levels were maintained below the clean water threshold for recirculating aquaculture of crab Eriocheir sinensis.Furthermore, the added strains exhibited high bio-safety and were capable of improving the yield and quality of crabs.Results indicate the here potential applicability of biochar-immobilized Sphingomonas sp.PDD-57b-25 and A.towneri in agricultural sewage treatments.
Further, the experimental methodology developed here may be used for the exploration of new strains for practical aquaculture.